A microspectrophotometric study of Papanicolaou-stained cervical cells as an aid in computerized image processing.

@article{Holmquist1976AMS,
  title={A microspectrophotometric study of Papanicolaou-stained cervical cells as an aid in computerized image processing.},
  author={John Holmquist and Y Imasoto and Ewert Bengtsson and Bj{\"o}rn Olsen and B. Stenkvist},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1976},
  volume={24 12},
  pages={1218-24}
}
As part of a study of cytologic automation, microspectrophotometric investigation of Papanicolaou-stained cervical cells was performed, using a Leitz MPV-II scanning photometer connected to a PDP 8/F minicomputer. It was shown that the selection of one single wavelength may result in difficulties in detecting boundries between background and cytoplasm and/or between cytoplasm nucleus. A set of two wavelengths, 530 nm and 570 nm, were found to be optimal for the image processing of these cells. 

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