A microscope-based flow cytophotometer

@article{Steen1983AMF,
  title={A microscope-based flow cytophotometer},
  author={Harald Steen},
  journal={The Histochemical Journal},
  year={1983},
  volume={15},
  pages={147-160}
}
By means of a new flow chamber, a standard fluorescence microscope with Epi illumination and 100 W mercury arc excitation has been turned into a flow cytophotometer combining high resolution and sensitivity with simplicity of operation. In the flow chamber, cells are passed in a narrow stream through the microscope focus carried by a laminar flow of water running on the open surface of a cover glass which is coupled to the oil immersion microscope objective. Two spectral components of the… CONTINUE READING