A microplate assay for the detection of oxidative products using 2',7'-dichlorofluorescin-diacetate.

  title={A microplate assay for the detection of oxidative products using 2',7'-dichlorofluorescin-diacetate.},
  author={Alexander R. Rosenkranz and Sabine Schmaldienst and Karl Manfred Stuhlmeier and Weisan Chen and Walter E Knapp and Gerhard J Zlabinger},
  journal={Journal of immunological methods},
  volume={156 1},
A fluorometric microplate assay was established for the detection of respiratory burst activity in phagocytic cells by assessing oxidation of 2',7'-dichlorofluorescin-diacetate (DCFH-DA). This method is based on flow cytometric studies by Bass et al. (J. Immunol. 130 (1983) p. 1910) describing intracellular detection of DCFH oxidation due to the presence of hydrogen peroxides. In the present study we have adapted the assay for use in microtiter plates to determine the amount of extracellular… CONTINUE READING
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