A micrococcal nuclease homologue in RNAi effector complexes

@article{Caudy2003AMN,
  title={A micrococcal nuclease homologue in RNAi effector complexes},
  author={Amy A. Caudy and Ren{\'e} F. Ketting and Scott M. Hammond and Ahmet M. Denli and Anja M. P. Bathoorn and Bastiaan B.J. Tops and Jose M. Silva and Mike Myers and Gregory J. Hannon and Ronald H. A. Plasterk},
  journal={Nature},
  year={2003},
  volume={425},
  pages={411-414}
}
RNA interference (RNAi) regulates gene expression by the cleavage of messenger RNA, by mRNA degradation and by preventing protein synthesis. These effects are mediated by a ribonucleoprotein complex known as RISC (RNA-induced silencing complex). We have previously identified four Drosophila components (short interfering RNAs, Argonaute 2 (ref. 2), VIG and FXR) of a RISC enzyme that degrades specific mRNAs in response to a double-stranded-RNA trigger. Here we show that Tudor-SN (tudor… 
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The RISC subunit Tudor-SN binds to hyper-edited double-stranded RNA and promotes its cleavage
  • A. Scadden
  • Biology
    Nature Structural &Molecular Biology
  • 2005
TLDR
It is shown that Tudor-SN specifically interacts with and promotes cleavage of model hyper-edited dsRNA substrates containing multiple I·U and U·I pairs, suggesting a novel unsuspected interplay between the two pathways that is more complex than mutual antagonism.
RNA Silencing Pathways in Schizosaccharomyces pombe and Drosophila melanogaster : A Dissertation
TLDR
The Schizosaccharomyces pombe genome encodes only one of each of the three major classes of proteins implicated in RNA silencing: Dicer, RdRP and Argonaute, and these three proteins are required for silencing at centromeres and for the initiation of transcriptionally silent heterochromatin at the mating-type locus.
Biochemical Mechanism of RNA Interference in Higher Organisms: A Dissertation
TLDR
Designing siRNAs that target the single nucleotide polymorphism in superoxide dismutase that causes the familial form of amyotrophic lateral sclerosis (ALS), but leave the wild-type mRNA intact and functional is used.
Endogenous antiviral mechanisms of RNA interference: a comparative biology perspective.
TLDR
The literature in this field is reviewed, which may open doors to the many uses to which this important technology is being put, including the potential of RNAi as a therapeutic strategy for gene regulation to modulate host-pathogen interactions.
The RNA-Induced Silencing Complex Is a Mg2+-Dependent Endonuclease
Uncoupling of RNAi from active translation in mammalian cells.
TLDR
The results demonstrate that neither active translation nor unidirectional scanning is required for siRNA mediated target degradation, and nontranslated mRNAs are highly susceptible to RNAi, and blocking scanning from both the 5' and 3' ends of an mRNA does not impede RNAi.
Induction of RNA interference genes by double-stranded RNA; implications for susceptibility to RNA interference.
An ortholog of the Vasa intronic gene is required for small RNA-mediated translation repression in Chlamydomonas reinhardtii
TLDR
Chlamydomonas VIG1, an ortholog of the Drosophila melanogaster Vasa intronic gene (VIG), is required for the repression of sRNA-targeted transcripts at the translational level but is dispensable for cleavage-mediated RNA interference and for the association of the AGO3 effector with polyribosomes or target transcripts.
Tudor staphylococcal nuclease is a structure-specific ribonuclease that degrades RNA at unstructured regions during microRNA decay.
TLDR
This study provides the molecular basis for how TSN cooperates with RNA editing to eliminate duplex RNA in cell defense, andHow TSN selects and degrades RNA during microRNA decay.
Penaeus monodon Tudor staphylococcal nuclease preferentially interacts with N-terminal domain of Argonaute-1.
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References

SHOWING 1-10 OF 36 REFERENCES
Role for a bidentate ribonuclease in the initiation step of RNA interference
TLDR
Dicer is a member of the RNase III family of nucleases that specifically cleave double-stranded RNAs, and is evolutionarily conserved in worms, flies, plants, fungi and mammals, and has a distinctive structure, which includes a helicase domain and dualRNase III motifs.
Argonaute2, a Link Between Genetic and Biochemical Analyses of RNAi
TLDR
Biochemical purification of the RNAi effector nuclease from cultured Drosophila cells reveals that one constituent of this complex is a member of the Argonaute family of proteins, which are essential for gene silencing in Caenorhabditis elegans, Neurospora, and Arabidopsis.
An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells
TLDR
It is shown that ‘loss-of-function’ phenotypes can be created in cultured Drosophila cells by transfection with specific double-stranded RNAs, which coincides with a marked reduction in the level of cognate cellular messenger RNAs.
Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans.
TLDR
A combination of phenotypic abnormalities and RNA analysis suggests a role for dcr-1 in a regulatory pathway comprised of small temporal RNA (let-7) and its target (e.g., lin-41).
Genes and Mechanisms Related to RNA Interference Regulate Expression of the Small Temporal RNAs that Control C. elegans Developmental Timing
Fragile X-related protein and VIG associate with the RNA interference machinery.
TLDR
The characterization of RNAi effector complexes (RISCs) that contain small interfering RNAs and microRNAs (miRNAs) and the possibility that dFXR, and potentially FMRP, use, at least in part, an RNAi-related mechanism for target recognition suggests a potentially important link between RNAi and human disease.
A biochemical framework for RNA silencing in plants.
TLDR
This finding supports the view that plant miRNAs direct RNAi and that miRNA-specified mRNA destruction is important for proper plant development and endonuclease complexes guided by small RNAs are a common feature of RNA silencing in both animals and plants.
Single-Stranded Antisense siRNAs Guide Target RNA Cleavage in RNAi
A microRNA in a Multiple-Turnover RNAi Enzyme Complex
TLDR
It is shown that, in human cell extracts, the miRNA let-7 naturally enters the RNAi pathway, which suggests that only the degree of complementarity between a miRNA and its RNA target determines its function.
Inhibition of HIV-1 Infection by Small Interfering RNA-Mediated RNA Interference1
TLDR
It is demonstrated that RNAi mediated by 21-bp dsRNA specifically inhibits HIV-1 infection of permanent cell lines and primary CD4+ T cells and derivatized siRNA could be delivered without lipofectin complexing and in the presence of serum.
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