A microchamber array for single cell isolation and analysis of intracellular biomolecules.

@article{Eyer2012AMA,
  title={A microchamber array for single cell isolation and analysis of intracellular biomolecules.},
  author={Klaus Eyer and Phillip Kuhn and Conni Hanke and Petra Stephanie Dittrich},
  journal={Lab on a chip},
  year={2012},
  volume={12 4},
  pages={765-72}
}
We present a microfluidic device that enables the determination of intracellular biomolecules in multiple single cells. The cells are individually trapped and isolated in a microchamber array. Since the microchambers can be opened and closed reversibly, the cells can be exposed to different solutions sequentially, e.g. for incubation, washing steps, labelling and finally, for lysis. The tightly sealed microchambers enable the retention and analysis of cell lysate derived from single cells. The… CONTINUE READING

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