A micro thin layer chromatography technique for urinary and serum amino acids using fluorescamine as a staining reagent

@article{Wang1983AMT,
  title={A micro thin layer chromatography technique for urinary and serum amino acids using fluorescamine as a staining reagent},
  author={Tso-Ren Wang and Ming-liang Lee and David A Toke},
  journal={Japanese journal of human genetics},
  year={1983},
  volume={28},
  pages={273-284}
}
SummaryA rapid, sensitive, inexpensive micro technique for thin layer chromatography of amino acids from urine and blood is described. The method which requires only 0.1 μl of sample, uses a fluorescent reagent, fluorescamine, as a staining agent, and commercially available cellulose plates, Polygram Cel 300, as a supporting matrix. The great majority of amino acids can be detected when concentration is greater than 0.25mm. The application of this technique toward the identification of various… CONTINUE READING

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