A methodology for markerless genetic modifications in Azotobacter vinelandii.

Abstract

AIMS Efficient manipulation of multiple regions within a genome can be improved by counter-selection approaches. In this work, we sought to develop a method to manipulate Azotobacter vinelandii using a counter-selection approach based on the presence of the pyrF gene. METHODS AND RESULTS A background uracil auxotroph of A. vinelandii was first constructed… (More)
DOI: 10.1111/jam.13091

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