A method to stain nuclei of Drosophila for confocal microscopy.

  title={A method to stain nuclei of Drosophila for confocal microscopy.},
  author={Sandra Orsulic and Mark Peifer},
  volume={16 3},
We report a method of staining nurse cell and follicle cell nuclei in Drosophila ovaries and nuclei in Drosophila embryos with the fluorescent dye propidium iodide. This technique was used to replace more commonly used 4', 6-diamidino-2-phenylindole (DAPI) and Hoechst staining as a method of visualizing nuclear material in Drosophila. Propidium iodide has its maximum excitation at about 530 nm and maximum fluorescence at 615 nm, and therefore it can be used as a fluorescent marker with confocal… CONTINUE READING


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