A method to preserve sputum for delayed examination.

@article{Kelly2003AMT,
  title={A method to preserve sputum for delayed examination.},
  author={Margaret M. Kelly and Frederick E. Hargreave and Gerard Cox},
  journal={The European respiratory journal},
  year={2003},
  volume={22 6},
  pages={996-1000}
}
Examination of sputum cell counts is limited by the need to process samples within hours of expectoration. The validity and repeatability of a method to preserve sputum for delayed processing and examination were investigated in this study. Portions of selected sputum from 39 subjects were dispersed with dithiothreitol (routine method), or were fixed in a dithiothreitol-formaldehyde mixture for 48-72 h before dispersal with trypsin (preservation method). Total and differential cell counts using… CONTINUE READING