A method to obtain avian germ-line chimaeras using isolated primordial germ cells.

@article{Yasuda1992AMT,
  title={A method to obtain avian germ-line chimaeras using isolated primordial germ cells.},
  author={Yoshiko Yasuda and Atsushi Tajima and Toyoshi Fujimoto and Takashi Kuwana},
  journal={Journal of reproduction and fertility},
  year={1992},
  volume={96 2},
  pages={521-8}
}
Primordial germ cells (PGCs), collected from the blood of 2-day-old chick embryos, were concentrated by Ficoll density centrifugation. The blood contained 0.048% PGCs and the concentrated fraction contained 3.9% PGCs in blood cells. The PGCs were picked up with a fine glass pipette, and one hundred were then injected into the terminal sinuses of 2-day-old Japanese quail embryos (24 somites); bubbles were then inserted to prevent haemorrhage. The embryos were further incubated at 38 degrees C… CONTINUE READING

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