A method to measure cardiac autophagic flux in vivo.

@article{IwaiKanai2008AMT,
  title={A method to measure cardiac autophagic flux in vivo.},
  author={Eri Iwai-Kanai and Hua Yuan and Chengqun Huang and M. Richard Sayen and Cynthia N Perry-Garza and Lucy M. Kim and Roberta A Gottlieb},
  journal={Autophagy},
  year={2008},
  volume={4 3},
  pages={322-9}
}
Autophagy, a highly conserved cellular mechanism wherein various cellular components are broken down and recycled through lysosomes, has been implicated in the development of heart failure. However, tools to measure autophagic flux in vivo have been limited. Here, we tested whether monodansylcadaverine (MDC) and the lysosomotropic drug chloroquine could be used to measure autophagic flux in both in vitro and in vivo model systems. Using HL-1 cardiac-derived myocytes transfected with GFP-tagged… CONTINUE READING