A method to detect ras point mutations in small subpopulations of cells.

@article{Chen1991AMT,
  title={A method to detect ras point mutations in small subpopulations of cells.},
  author={J Chen and Michael V. Viola},
  journal={Analytical biochemistry},
  year={1991},
  volume={195 1},
  pages={51-6}
}
Biochemical assays for ras mutations are capable of detecting a mutant allele only if it is present in at least 5% of cells tested. Further, ras mutation assays which utilize the polymerase chain reaction (PCR) are unable to distinguish a ras mutation in a small population of cells from mutations resulting from Taq DNA polymerase base misincorporation. We used a standard restriction fragment length polymorphism assay of PCR-amplified c-Ki-ras to detect codon 12 mutations in tumor cells and… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 17 extracted citations

Clinical and Biological Basis of Lung Cancer Prevention

Respiratory Pharmacology and Pharmacotherapy • 1998

Similar Papers

Loading similar papers…