A method of preparing blood leucocytes for flow cytometry which prevents upregulation of leucocyte integrins.

@article{Hamblin1992AMO,
  title={A method of preparing blood leucocytes for flow cytometry which prevents upregulation of leucocyte integrins.},
  author={Anne S. Hamblin and Michael J. Taylor and J{\"u}rgen Bernhagen and Z Shakoor and S. Mayall and G. Noble and D. Mccarthy},
  journal={Journal of immunological methods},
  year={1992},
  volume={146 2},
  pages={
          219-28
        }
}
LeuCAM (CD11/CD18) cell-surface antigens are easily upregulated on cell manipulation ex vivo. A procedure for preparing leucocytes, in which human blood is immediately treated ex vivo with buffered formaldehyde and then the erythrocytes and platelets are removed by lysis and differential centrifugation, has been successfully applied to the analysis of LeuCAM antigen expression by flow cytometry. We show that the increased expression of monocyte CD11/CD18, which occurs when mononuclear… CONTINUE READING

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