A method of high frequency virus-induced gene silencing in chili pepper (Capsicum annuum L. cv. Bukang).

@article{Chung2004AMO,
  title={A method of high frequency virus-induced gene silencing in chili pepper (Capsicum annuum L. cv. Bukang).},
  author={Eunsook Chung and Eunsoo Seong and Yeoung-Cheol Kim and Eun Joo Chung and Sang-Keun Oh and Sanghyeob Lee and Jeong Mee Park and Young Hee Joung and Doil Choi},
  journal={Molecules and cells},
  year={2004},
  volume={17 2},
  pages={377-80}
}
Using a tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) system, expression of phytogene desaturase (PDS) and ribulose-1,5-bisphosphate carboxylase small-subuit (rbcS) genes was suppressed in Nicotiana benthamiana and pepper plants (Capsicum annuum L. cv. Bukang). The silenced phenotypes of pale yellow (rbcS), and photobleached leaves (PDS), were invariably obvious 2 weeks after inoculation with the TRV-based vector. In a parallel experiment, the same set of genes was… CONTINUE READING

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