A method for the production and expedient screening of CRISPR/Cas9-mediated non-transgenic mutant plants

@inproceedings{Chen2018AMF,
  title={A method for the production and expedient screening of CRISPR/Cas9-mediated non-transgenic mutant plants},
  author={Longzheng Chen and Wei Li and Lorenzo Katin-Grazzini and Jing Bin Ding and Xianbin Gu and Yanjun Li and Tingting Gu and Ren Qing Wang and Xinchun Lin and Ziniu N. Deng and Richard J. McAvoy and Frederick G Gmitter and Zhanao Deng and Yunde Zhao and Yi Li},
  booktitle={Horticulture Research},
  year={2018}
}
Developing CRISPR/Cas9-mediated non-transgenic mutants in asexually propagated perennial crop plants is challenging but highly desirable. Here, we report a highly useful method using an Agrobacterium-mediated transient CRISPR/Cas9 gene expression system to create non-transgenic mutant plants without the need for sexual segregation. We have also developed a rapid, cost-effective, and high-throughput mutant screening protocol based on Illumina sequencing followed by high-resolution melting (HRM… CONTINUE READING
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