A method for the preparation of protein-protein conjugates of predetermined composition.

@article{Rector1978AMF,
  title={A method for the preparation of protein-protein conjugates of predetermined composition.},
  author={Edward S. Rector and R J Schwenk and K. S. Maurice Tse and Alec H. Sehon},
  journal={Journal of immunological methods},
  year={1978},
  volume={24 3-4},
  pages={321-36}
}
A novel procedure for the synthesis of well-defined protein-protein conjugates is described using ovalbumin (OA) and IgG as test proteins. This procedure involves the highly selective and rapid reaction of alkyl halide and sulfhydryl groups, which have been grafted, respectively, onto the proteins to be conjugated. Accordingly, iodoacetylated IgG, (ICH2CO)nIgG, was prepared by the reaction of the epsilon-amino groups of IgG with the N-hydroxysuccinimide ester of iodoacetic acid (NHIA), the… CONTINUE READING