A method for the isolation of longevity mutants in the nematode Caenorhabditis elegans and initial results

@article{Klass1983AMF,
  title={A method for the isolation of longevity mutants in the nematode Caenorhabditis elegans and initial results},
  author={Michael R. Klass},
  journal={Mechanisms of Ageing and Development},
  year={1983},
  volume={22},
  pages={279-286}
}
  • M. Klass
  • Published 1 July 1983
  • Biology, Medicine
  • Mechanisms of Ageing and Development
The free-living nematode Caenorhabditis elegans is used as a genetically manipulable experimental system for the study of aging. Utilizing a temperature-sensitive sterile strain with a normal life span, a method is described for the isolation of mutant strains with significantly increased life spans. Eight mutant strains were isolated each having increased life spans. Two mutant strains were spontaneous dauer formers, accounting for their increased longevity. Another was chemotaxis-defective… Expand

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References

SHOWING 1-10 OF 24 REFERENCES
Aging in the nematode Caenorhabditis elegans: Major biological and environmental factors influencing life span
  • M. Klass
  • Medicine, Biology
  • Mechanisms of Ageing and Development
  • 1977
TLDR
The present study identifies some of the major biological and environmental factors influencing life span as a prelude to more detailed genetic and biochemical analyses in Caenorhabditis elegans. Expand
Nondisjunction Mutants of the Nematode CAENORHABDITIS ELEGANS.
TLDR
The frequency of males among the self progeny of wild-type Caenorhabditis elegans hermaphrodites (5AA; XX) is about one in 500, and fifteen him mutations have been identified that increase this frequency by a factor of ten to 150, as a result of increased X-chromosome nondisjunction. Expand
The genetics of Caenorhabditis elegans.
TLDR
Estimates of the induced mutation frequency of both the visible mutants and X chromosome lethals suggests that, just as in Drosophila, the genetic units in C. elegans are large. Expand
Non-ageing developmental variant of Caenorhabditis elegans
TLDR
Larval forms of the free-living soil nema-tode, Caenorhabditis elegans, possess the ability to enter a semi-dormant, quiescent state referred to as the dauer larval stage (German, “enduring” larva), which enables the species to retard its ageing rate and extend its life span. Expand
NONDISJUNCTION MUTANTS OF THE NEMATODE
TLDR
Fifteen him (for “high incidence of males”) mutations have been identified that increase this frequency by a factor of ten to 150, as a result of increased X-chromosome nondisjunction. Expand
The dauerlarva, a post-embryonic developmental variant of the nematode Caenorhabditis elegans.
TLDR
It has been shown that the dauerlarva is formed by a facultative, reversible arrest at a specific point in the life cycle, the second of four cuticle molts, in response to external conditions. Expand
Post-embryonic cell lineages of the nematode, Caenorhabditis elegans.
TLDR
These cell lineages range in length from one to eight sequential divisions and lead to significant developmental changes in the neuronal, muscular, hypodermal, and digestive systems and are determined by direct observation of the divisions, migrations, and deaths of individual cells in living nematodes. Expand
Cell lineages of the embryo of the nematode Caenorhabditis elegans.
TLDR
The anterior position of the site of origin of the stem cells in the egg relates to the rate of the cell cycle clock, suggesting intracellular preprogramming of the uncleaved egg. Expand
Superoxide dismutase activity in dauerlarvae of Caenorhabditis elegans (Nematoda: Rhabditidae)
TLDR
This study characterizes superoxide dismutase (SODase) activity in several developmental stages of Caenorhabditis elegans (originally described by E. Maupas in 1900) and suggests the elevation of this activity relative to metabolic rate could account for the long life-span of dauerlarvae. Expand
Sperm isolation and biochemical analysis of the major sperm protein from Caenorhabditis elegans.
TLDR
Biochemical analyses show that sperm contain a major protein component that represents 17% of the total sperm protein, and Poly(A) mRNA coding for this protein is first detected in a translatable form just before synthesis of this sperm protein suggesting transcriptional control. Expand
...
1
2
3
...