A method for the generation of conditional gene repair mutations in mice.

@article{Dragatsis2001AMF,
  title={A method for the generation of conditional gene repair mutations in mice.},
  author={Ioannis Dragatsis and Scott Zeitlin},
  journal={Nucleic acids research},
  year={2001},
  volume={29 3},
  pages={E10}
}
Conditional gene repair mutations in the mouse can assist in cell lineage analyses and provide a valuable complement to conditional gene inactivation strategies. We present a method for the generation of conditional gene repair mutations that employs a loxP-flanked (floxed) selectable marker and transcriptional/translational stop cassette (neostop) located within the first intron of a target gene. In the absence of Cre recombinase, expression of the targeted allele is suppressed generating a… CONTINUE READING