A method for the determination of hyaluronate in the presence of other glycosaminoglycans and its application to human intervertebral disc.

@article{Hardingham1976AMF,
  title={A method for the determination of hyaluronate in the presence of other glycosaminoglycans and its application to human intervertebral disc.},
  author={Tim E. Hardingham and Paul Adams},
  journal={The Biochemical journal},
  year={1976},
  volume={159 1},
  pages={143-7}
}
A highly sensitive proteoglycan-binding assay was developed for the detection and determination of small amounts of hyaluronate. Application of this assay to human intervertebral-disc tissue showed that the radial distribution of hyaluronate closely followed the distribution of proteoglycans. 

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References

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Chemistry and Molecular Biology of the Extracellular Matrix (Balazs

  • J. S. Brimacombe, J. M. Webber
  • Biochim. Biophys. Acta
  • 1975

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