A method for the detection and quantification of bacteria in human carious dentine using fluorescent in situ hybridisation.

@article{Banerjee2002AMF,
  title={A method for the detection and quantification of bacteria in human carious dentine using fluorescent in situ hybridisation.},
  author={Avijit Banerjee and M Yasseri and Margaret Munson},
  journal={Journal of dentistry},
  year={2002},
  volume={30 7-8},
  pages={359-63}
}
OBJECTIVES Previous studies have evaluated bacterial numbers in carious dentine using conventional culturing methods, capable of detecting only a proportion of the total bacteria present within lesions. The aim of this study was to detect and enumerate the total bacterial population present in carious human dentine by means of fluorescent in situ hybridisation. METHOD Five freshly extracted carious molars were sequentially hand excavated under sterile conditions through four levels in the… CONTINUE READING