A method for the detection and differentiation of cellulase components in polyacrylamide gels.

@article{Bartley1984AMF,
  title={A method for the detection and differentiation of cellulase components in polyacrylamide gels.},
  author={Timothy D. Bartley and K Murphy-Holland and Douglas E. Eveleigh},
  journal={Analytical biochemistry},
  year={1984},
  volume={140 1},
  pages={157-61}
}
Endoglucanase and exoglucanase components of cellulase can be detected and differentiated after polyacrylamide gel electrophoresis by performing activity stains. Endoglucanase activity was visualized in carboxymethyl cellulose agar replicas of gels by staining with Congo red. General beta-1,4-glucanase activity was located by soaking the gel in a solution of NaBH4-reduced cellulooligosaccharides, and detecting the formation of reducing sugars by reaction with triphenyl tetrazolium chloride… CONTINUE READING