A method for the comprehensive proteomic analysis of membrane proteins

@article{Wu2003AMF,
  title={A method for the comprehensive proteomic analysis of membrane proteins},
  author={Christine C. Wu and Michael J. MacCoss and Kathryn E. Howell and John R. Yates},
  journal={Nature Biotechnology},
  year={2003},
  volume={21},
  pages={532-538}
}
We describe a method that allows for the concurrent proteomic analysis of both membrane and soluble proteins from complex membrane-containing samples. When coupled with multidimensional protein identification technology (MudPIT), this method results in (i) the identification of soluble and membrane proteins, (ii) the identification of post-translational modification sites on soluble and membrane proteins, and (iii) the characterization of membrane protein topology and relative localization of… CONTINUE READING

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