A method for the comprehensive proteomic analysis of membrane proteins

  title={A method for the comprehensive proteomic analysis of membrane proteins},
  author={Christine C. Wu and Michael J. MacCoss and Kathryn E. Howell and John R. Yates},
  journal={Nature Biotechnology},
We describe a method that allows for the concurrent proteomic analysis of both membrane and soluble proteins from complex membrane-containing samples. When coupled with multidimensional protein identification technology (MudPIT), this method results in (i) the identification of soluble and membrane proteins, (ii) the identification of post-translational modification sites on soluble and membrane proteins, and (iii) the characterization of membrane protein topology and relative localization of… CONTINUE READING


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