A method for site-specific labeling of multiple protein thiols.

Abstract

We present a generic method for the site-specific and differential labeling of multiple cysteine residues in one protein. Phenyl arsenic oxide has been employed as a protecting group of two closely spaced thiols, allowing first labeling of a single thiol. Subsequently, the protecting group is removed, making available a reactive dithiol site for labeling… (More)
DOI: 10.1002/pro.113

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