A method for rapid production of heteromultimeric protein complexes in plants: assembly of protective bluetongue virus-like particles.

@article{Thuenemann2013AMF,
  title={A method for rapid production of heteromultimeric protein complexes in plants: assembly of protective bluetongue virus-like particles.},
  author={Eva C. Thuenemann and Ann E Meyers and Jeanette Verwey and Edward Peter Rybicki and G. P. Lomonossoff},
  journal={Plant biotechnology journal},
  year={2013},
  volume={11 7},
  pages={839-46}
}
Plant expression systems based on nonreplicating virus-based vectors can be used for the simultaneous expression of multiple genes within the same cell. They therefore have great potential for the production of heteromultimeric protein complexes. This work describes the efficient plant-based production and assembly of Bluetongue virus-like particles (VLPs), requiring the simultaneous expression of four distinct proteins in varying amounts. Such particles have the potential to serve as a safe… CONTINUE READING
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