A method for preparing 2- to 50-µm-thick fresh-frozen sections of large samples and undecalcified hard tissues

@article{Kawamoto2000AMF,
  title={A method for preparing 2- to 50-µm-thick fresh-frozen sections of large samples and undecalcified hard tissues},
  author={Tadafumi Kawamoto and Masaharu Shimizu},
  journal={Histochemistry and Cell Biology},
  year={2000},
  volume={113},
  pages={331-339}
}
This article describes a method for preparing 2- to 50-µm-thick fresh-frozen sections from large samples and completely calcified tissue samples. In order to perform the more routine work involved, a tungsten carbide disposable blade was installed to a heavy-duty sledge cryomicrotome. An entire 10-day-old rat and bone and tooth samples from a 7-month-old… CONTINUE READING