A method for observing intracellular structures of free cells by scanning electron microscopy.


A new method is described for scanning electron microscopic observation of cultured free cells using chitosan embedding. Intracellular structures of free cells can be clearly observed using a combination of the chitosan embedding and the Osmium-DMSO-Osmium (O-D-O) method. Chitosan, a polysaccharide, is not affected by the osmium maceration procedure central… (More)