A method for mapping the distribution pattern of cariogenic streptococci within dental plaque in vivo.

@article{Kato2004AMF,
  title={A method for mapping the distribution pattern of cariogenic streptococci within dental plaque in vivo.},
  author={Kazuo Kato and Toru Sato and Nobuhiro Takahashi and Keiko Fukui and Kyoko Yamamoto and Haruo Nakagaki},
  journal={Caries research},
  year={2004},
  volume={38 5},
  pages={
          448-53
        }
}
This study was carried out to develop a method for mapping the distribution of cariogenic oral streptococci, Streptococcus mutans and Streptococcus sobrinus, from the outermost to the innermost plaque. Ten consenting subjects were asked to form plaque by abstaining from tooth brushing over 3 days within in situ plaque-generating devices, which were placed on the upper molars. The plaque formed in the devices was separated into 8-10 layered fractions (100 microm thick). Genomic DNA was extracted… CONTINUE READING