A method for isolation of plasmid DNA replication intermediates from unsynchronized bacterial cultures for electron microscopy analysis.

Abstract

Electron microscopy is a powerful technique for analysis of DNA replication intermediates. However, isolation of replicating DNA molecules from living cells is tricky and difficult, especially in the case of small DNA molecules (such as bacterial plasmids) whose initiation of replication is not easily synchronized. Here a relatively simple and rapid method… (More)

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@article{Srutkowska1998AMF, title={A method for isolation of plasmid DNA replication intermediates from unsynchronized bacterial cultures for electron microscopy analysis.}, author={S. Srutkowska and Grażyna Konopa and Grace H Wegrzyn}, journal={Acta biochimica Polonica}, year={1998}, volume={45 1}, pages={233-40} }