A method for high purity sorting of rare cell subsets applied to TDC.

@article{Kuka2013AMF,
  title={A method for high purity sorting of rare cell subsets applied to TDC.},
  author={Mirela Kuka and Jonathan D Ashwell},
  journal={Journal of immunological methods},
  year={2013},
  volume={400-401},
  pages={111-6}
}
T(DC) are a recently described subset of polyclonal αβ T-cells with dendritic cell properties. Because of their low number in peripheral immune compartments, isolation and characterization of T(DC) with existing purification methods are technically challenging. Here we describe a customized gating strategy and a flow cytometry-based cell sorting protocol… CONTINUE READING