A method for growing a biofilm under low shear at the air–liquid interface using the drip flow biofilm reactor

@article{Goeres2009AMF,
  title={A method for growing a biofilm under low shear at the air–liquid interface using the drip flow biofilm reactor},
  author={Darla M Goeres and Martin Alva Hamilton and Nicholas A. Beck and Kelli A Buckingham-Meyer and Jackie D Hilyard and Linda R Loetterle and Lindsey A Lorenz and Diane K Walker and Philip S Stewart},
  journal={Nature Protocols},
  year={2009},
  volume={4},
  pages={783-788}
}
This protocol describes how to grow a Pseudomonas aeruginosa biofilm under low fluid shear close to the air–liquid interface using the drip flow reactor (DFR). The DFR can model environments such as food-processing conveyor belts, catheters, lungs with cystic fibrosis and the oral cavity. The biofilm is established by operating the reactor in batch mode for 6 h. A mature biofilm forms as the reactor operates for an additional 48 h with a continuous flow of nutrients. During continuous flow, the… CONTINUE READING

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