A method for genetic modification of human embryonic stem cells using electroporation

@article{Costa2007AMF,
  title={A method for genetic modification of human embryonic stem cells using electroporation},
  author={Magdaline Costa and Mirella Dottori and Koula Sourris and Pegah Jamshidi and Tanya Hatzistavrou and Richard Dean Davis and Lisa Azzola and Steven J. Jackson and Sue Mei Lim and Martin F Pera and Andrew G. Elefanty and Edouard G. Stanley},
  journal={Nature Protocols},
  year={2007},
  volume={2},
  pages={792-796}
}
The ability to genetically modify human embryonic stem cells (HESCs) will be critical for their widespread use as a tool for understanding fundamental aspects of human biology and pathology and for their development as a platform for pharmaceutical discovery. Here, we describe a method for the genetic modification of HESCs using electroporation, the preferred method for introduction of DNA into cells in which the desired outcome is gene targeting. This report provides methods for cell… CONTINUE READING