A method for genetic modification of human embryonic stem cells using electroporation

@article{Costa2007AMF,
  title={A method for genetic modification of human embryonic stem cells using electroporation},
  author={M. Costa and M. Dottori and K. Sourris and Pegah Jamshidi and T. Hatzistavrou and R. Davis and Lisa Azzola and S. Jackson and S. M. Lim and M. Pera and A. Elefanty and E. Stanley},
  journal={Nature Protocols},
  year={2007},
  volume={2},
  pages={792-796}
}
  • M. Costa, M. Dottori, +9 authors E. Stanley
  • Published 2007
  • Biology, Medicine
  • Nature Protocols
  • The ability to genetically modify human embryonic stem cells (HESCs) will be critical for their widespread use as a tool for understanding fundamental aspects of human biology and pathology and for their development as a platform for pharmaceutical discovery. Here, we describe a method for the genetic modification of HESCs using electroporation, the preferred method for introduction of DNA into cells in which the desired outcome is gene targeting. This report provides methods for cell… CONTINUE READING

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