A method for enzymatic determination of citrate in serum and urine.

@article{Nielsen1976AMF,
  title={A method for enzymatic determination of citrate in serum and urine.},
  author={T Toftegaard Nielsen},
  journal={Scandinavian journal of clinical and laboratory investigation},
  year={1976},
  volume={36 6},
  pages={513-9}
}
A method for determination of citrate in serum and urine using citrate lyase is described. The influence of pH, zinc, magnesium, and calcium ions on the reaction velocity is studied. Citrate lyase activity is found rather insensitive to variations in pH round the pH-optimum at 8.2. Zinc ions activate the reactions. The optimal concentration of zinc ions in the reaction mixture is found to depend on the calcium concentration of samples. Perchloric acid is used for precipitation of proteins. The… CONTINUE READING