A method for direct cloning of fur-regulated genes: identification of seven new fur-regulated loci in Escherichia coli.

@article{Vassinova2000AMF,
  title={A method for direct cloning of fur-regulated genes: identification of seven new fur-regulated loci in Escherichia coli.},
  author={N Vassinova and D P Kozyrev},
  journal={Microbiology},
  year={2000},
  volume={146 Pt 12},
  pages={3171-82}
}
A strain that allows the cloning of Fur-regulated loci was constructed. The strain, named FUR-SEL1, contains a chromosomal fhuA'-'cat transcriptional fusion that is expressed from the Fur-regulated promoter, fhuA(p). Therefore, Fur boxes introduced on a multicopy plasmid can cause derepression of the fusion by titrating the Fur repressor and thereby confer chloramphenicol resistance, which can be used as a selectable phenotype for cloning Fur-regulated loci. However, a number of additional… CONTINUE READING