A method for differentiating human induced pluripotent stem cells toward functional cardiomyocytes in 96-well microplates

@article{Balafkan2020AMF,
  title={A method for differentiating human induced pluripotent stem cells toward functional cardiomyocytes in 96-well microplates},
  author={Novin Balafkan and Sepideh Mostafavi and Manja Schubert and Richard Siller and Kristina Xiao Liang and Gareth John Sullivan and Laurence A. Bindoff},
  journal={Scientific Reports},
  year={2020},
  volume={10}
}
The capacity of pluripotent stem cells both for self-renewal and to differentiate into any cell type have made them a powerful tool for studying human disease. Protocols for efficient differentiation towards cardiomyocytes using defined, serum-free culture medium combined with small molecules have been developed, but thus far, limited to larger formats. We adapted protocols for differentiating human pluripotent stem cells to functional human cardiomyocytes in a 96-well microplate format. The… 
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Methods Citations
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12 Citations

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It is found that differentiated cardiomyocytes have significantly lower level of mitochondria content compared to their original source, pluripotent stem cells, although the efficiency of ATP generation via mitochondria respiration increases.
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TLDR
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TLDR
When incubated with synthetic spike proteins of Sars-Cov-2, human-induced pluripotent stem cells (hiPSC), and a culture device made of an arrayed monolayer of cross-linked nanofibers, the damage of the spike protein on hiPSC-CMs is enhanced due to downregulated ACE2, chromatin margination, altered Connexin 43 expression, sarcomere disruption, and beating break.
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TLDR
This work is able to show that action potentials from the same cell site can be recorded over days without detrimental effects to the cell membrane, and contributes towards a non-invasive approach for cardiomyocyte functional maturation, as well as developmental, pathological and pharmacological studies.
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