A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

@article{Martin1961AMF,
  title={A method for determining the sedimentation behavior of enzymes: application to protein mixtures.},
  author={R. Martin and B. Ames},
  journal={The Journal of biological chemistry},
  year={1961},
  volume={236},
  pages={
          1372-9
        }
}
  • R. Martin, B. Ames
  • Published 1961
  • Medicine, Chemistry
  • The Journal of biological chemistry
During an investigation of the gene-enzyme relationships in histidine biosynthesis in Xalmonella typhimurium, it became desirable to determine, in crude extracts, the approximate molecular weights of several enzymes. We have found sucrose gradient centrifugation to be a suitable method for determining sedimentation coefficients of enzymes in protein mixtures. A variety of enzymes of known properties have been studied in the development of the method. Although the separation cell of Yphantis and… Expand
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References

Some observations on the correlation of genetic information with enzyme molecular weight are discussed
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  • 1960