A method for determination of tulipalin A and B concentrations in crude extracts of tulip tissues

  title={A method for determination of tulipalin A and B concentrations in crude extracts of tulip tissues},
  author={J. C. M. Beijersbergen},
  journal={Recueil des Travaux Chimiques des Pays-Bas},
  • J. Beijersbergen
  • Published 2 September 2010
  • Biology
  • Recueil des Travaux Chimiques des Pays-Bas
A method for determining the concentration of the fungitoxic lactones tulipalin A and B in crude extracts of various parts of the tulip is described. After a simple fractionation of the extracts on Dowex 50 W, the amounts of these compounds can be measured by UV spectrophotometry. 
Molecular diversity of tuliposide B-converting enzyme in tulip (Tulipa gesneriana): identification of the third isozyme with a distinct expression profile
Identification of TgTCEB-L explains the PosB-converting activity detected in tissues where T gTCEB1 and Tg TCEB-R transcripts could not be detected, indicating that tulip subtilizes the three TGTCEB isozymes depending on the tissue.
Molecular diversity of tuliposide B-converting enzyme in tulip (Tulipa gesneriana): identification of the root-specific isozyme
Tissue-specific isozymes are involved in the conversion of 6-tuliposide B, a major secondary metabolite in tulip, to antibacterial tulipalin B, which encodes a TCEB.
A Novel Lactone-Forming Carboxylesterase: Molecular Identification of a Tuliposide A-Converting Enzyme in Tulip1[W]
To the knowledge, TgTCEA is the first identified member of the lactone-forming carboxylesterases, specifically catalyzing intramolecular transesterification in the tulip defensive strategy.
Molecular Diversity of Tuliposide A-Converting Enzyme in the Tulip
Tuliposide A-converting enzyme (TCEA) catalyzes the conversion of 6-tuliposide A to its lactonized aglycon, tulipalin A, in the tulip (Tulipa gesneriana). The TgTCEA gene, isolated previously from
Environmentally benign process for the preparation of antimicrobial α-methylene-β-hydroxy-γ-butyrolactone (tulipalin B) from tulip biomass
An environmentally benign and facile process for the preparation of PaB using tulip biomass materials based on one-step enzyme reaction catalyzed by TCE without the use of petroleum-derived solvents is described.
Function and application of a non-ester-hydrolyzing carboxylesterase discovered in tulip
  • T. Nomura
  • Biology
    Bioscience, biotechnology, and biochemistry
  • 2017
This review describes the biochemical and physiological aspects of tulipalin biogenesis, and the diverse functions of plant carboxylesterases in the α/β-hydrolase fold superfamily.
Plants and Plant Products
Contact dermatitis from plants or plant products may occur by several mechanisms and reactions of mixed aetiology, for instance irritant reactions superimposed on allergic reactions, are often seen, while mechanical plus chemical irritant effects are evoked by, for example, stinging nettles.


Isolation and identification of α‐methylene‐butyrolactone, A fungitoxic substance from tulips
A fungitoxic substance has been isolated from extracts from the white skins of tulip bulbs, from the fleshy bulb scales and from the flower pistils. The compound was identified as
Structure determination of the allergenic agent, isolated from tulip bulbs†
Handling of tulip bulbs taking place in bulb nurseries often causes a typical allergic reaction of the skin (“tulip fingers”). It is the purpose of this paper to report the results of an
A fungitoxic substance extracted from tulips and its possible role as a protectant against disease
It was concluded that the fungus is not able to penetrate into the tulip bulb during the greater part of the growing season, and a protecting barrier was demonstrated to be present in the white skin.
Presence of a substance in the white skin of young tulip bulbs which inhibits growth ofFusarium oxysporum
  • B. Bergman
  • Biology
    Netherlands Journal of Plant Pathology
  • 2007
It is suggested that this substance is protecting the susceptible fleshy bulb scales during the greater part of the growth period and that its diminution during the final weeks enablesF.
Über Glykoside mit lacton-bildendem Aglykon, II. Über die Struktur der antibiotisch aktiven Substanzen der Tulpe (Tulipa gesneriana L.)
Aus Tulipa gesneriana L. wurden zwei antibiotisch aktive Glucoside, 1-Tuliposid A (14a) und 1-Tuliposid B (15a), isoliert, die Acyl-β-D-glucopyranoside der γ-Hydroxy-α-methylen-buttersaure bzw.