A method for detecting rash and fever illness-associated viruses using multiplex reverse transcription polymerase chain reaction.

@article{Matsushima2017AMF,
  title={A method for detecting rash and fever illness-associated viruses using multiplex reverse transcription polymerase chain reaction.},
  author={Yuki Matsushima and Tomomi Shimizu and Ikuko Doi and Fuminori Mizukoshi and Koo Nagasawa and Akihide Ryo and Hideaki Shimizu and Masae Kobayashi and Keiji Funatogawa and Noriko Nagata and Mariko Ishikawa and Ayako Komane and Nobuhiko Okabe and Y. Mori and Makoto Takeda and Hirokazu Kimura},
  journal={Microbiology and immunology},
  year={2017},
  volume={61 8},
  pages={337-344}
}
In this study, a new multiplex RT-PCR method for detecting various viral genes in patients with rash and fever illnesses (RFIs) was constructed. New primer sets were designed for detection of herpes simplex viruses 1 and 2 (HSV1 and 2), and Epstein-Barr virus (EBV). The newly designed and previously reported primer sets were used to detect 13 types of RFI… CONTINUE READING