A method for counting acid-fast bacteria.

@article{Shepard1968AMF,
  title={A method for counting acid-fast bacteria.},
  author={Charles C. Shepard and D. S. Mcrae},
  journal={International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association},
  year={1968},
  volume={36 1},
  pages={
          78-82
        }
}
A reliable, convenient, and accurate method for counting ac id-fast hacteria ( AFB) is essential for much work with Mycobacterium leprae. The procedure used , previously in the laboratory (~, 4 ) had two technical difficulties: (a) In order for the drops to spread satisfactorily the microscope slides had to have a uniform wetability of the correct degree; sometimes this requirement was difficult to sa tisfy and a satisfactory procedure for one batch of slides was sometimes unsatisfactory for… CONTINUE READING

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