A method for construction of long randomized open reading frames and polypeptides.

@article{Mandecki1990AMF,
  title={A method for construction of long randomized open reading frames and polypeptides.},
  author={Wlodek Mandecki},
  journal={Protein engineering},
  year={1990},
  volume={3 3},
  pages={
          221-6
        }
}
A method is presented for construction of randomized open reading frame sequences (ORFs) and gene libraries containing them. The building blocks for the ORFs were 75 bp long DNA fragments generated by cloning sequences from a single synthetic oligonucleotide preparation by bridge mutagenesis. The fragments had the property that, regardless of their orientation in the ligated product, the ORF of the construct was maintained. The heterogeneity of the ORFs resulted from the random ligation of 2000… CONTINUE READING
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