A method for construction of E. coli strains with multiple DNA insertions in the chromosome.

@article{Peredelchuk1997AMF,
  title={A method for construction of E. coli strains with multiple DNA insertions in the chromosome.},
  author={Michael Peredelchuk and George N. Bennett},
  journal={Gene},
  year={1997},
  volume={187 2},
  pages={231-8}
}
A system for construction of E. coli strains with multiple DNA insertions in the chromosome, based on elements of modules for site specific recombination of Tn1545 and phage lambda, has been developed. Circular non-replicating DNA fragments containing the transposon attachment site (attTn), an excisable cassette with a selectable marker, and a gene of interest integrate randomly into the chromosome of a host E. coli strain when provided with transposon integrase, Int-Tn (the host strain was… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 13 extracted citations

A mini-Mu transposon-based method for multiple DNA fragment integration into bacterial genomes

Applied Microbiology and Biotechnology • 2010
View 4 Excerpts
Highly Influenced

CRISPR/Cas9-based systems: taking recombineering to the next level

Iranian journal of pharmaceutical research : IJPR • 2016

References

Publications referenced by this paper.
Showing 1-10 of 25 references

Gene disruption in Cells were cured from the helper plasmid and transduc

P. P. Cherepanov, W. Wackernagel
1995
View 4 Excerpts
Highly Influenced

Experiments in Molecular Genetics, Cold Spring

J. H. Miller
1972
View 4 Excerpts
Highly Influenced

a heterologous gene at multiple locations in the Bacillus subtilis chromosome by replacement recombination

P. Trieu-Cuot, C. Carlier, C. Poyart-Salmeron, P. Courvalin
mutagenesis and cloning of genes frombiol • 1991
View 3 Excerpts
Highly Influenced

A general method for the consecutive integration of single copies

Kiel, J.A.K.W, A. M. ten Berge, P. Borger, G. Venema
1995

A general method for the consecutive integration of single copies of fusions

W. J.A.K., A. M. ten Berge, P. Borger, G. Venema
1995

Bacterial vaccine vectors and bacillus Calmette-Guérin.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America • 1995
View 1 Excerpt

Uncon - strained bacterial promiscuity : the Tn 916Tn 1545 family of conjuga -

M. Y. Peredelchuk, N. D. Fedorova, Y. V. Smirnov, V. G. Debabov
1995

Similar Papers

Loading similar papers…