A method for constructing radiation hybrid maps of whole genomes

  title={A method for constructing radiation hybrid maps of whole genomes},
  author={Michael A. Walter and Dominique J. Spillett and Philip Thomas and Jean Weissenbach and Peter N. Goodfellow},
  journal={Nature Genetics},
In radiation hybrid mapping, chromosomes in human–rodent hybrid cells are fragmented by X–rays and fragments rescued by fusion of the donor cell to a recipient rodent cell. The co–retention frequencies of markers in 100–200 hybrids are used to map individual chromosomes, but mapping the whole genome in this way is impractical. We have reverted to the original protocols of Goss and Harris and have produced a panel of 44 hybrids using irradiated human fibroblasts as donors. This panel has been… CONTINUE READING

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