A membrane-bound fluorescent probe to detect phospholipid vesicle-cell fusion

  title={A membrane-bound fluorescent probe to detect phospholipid vesicle-cell fusion},
  author={C. Owen},
  journal={The Journal of Membrane Biology},
  • C. Owen
  • Published 2005
  • Chemistry, Medicine
  • The Journal of Membrane Biology
SummaryPyrenesulfonylphosphatidylethanolamine has been incorporated into sonicated phospholipid vesicles to provide a fluorescent signal from a membrane-bound probe whose spectrum is sensitive to the local concentration of dye molecules. When vesicle material was taken up by viable mouse splenocytes, the disappearance of the pyrene excimer fluorescence emission peak that accompanied dilution of the vesicle membrane lipid could be quantitated. One can thus measure, by a simple and rapid… 
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