A matrix for DNA separation: genotyping and sequencing using poly(vinylpyrrolidone) solution in uncoated capillaries.

Abstract

We report a new sieving matrix for DNA separation based on commercially available poly(vinylpyrrolidone). The new sieving matrix has a very low viscosity at moderate concentrations, e.g., 27 cP at 4.5%. Its excellent self-coating property can reduce electroosmotic flow to a negligible level. Column regeneration between runs is very simple and effective… (More)

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