A mass-tolerant database search identifies a large proportion of unassigned spectra in shotgun proteomics as modified peptides.


Fewer than half of all tandem mass spectrometry (MS/MS) spectra acquired in shotgun proteomics experiments are typically matched to a peptide with high confidence. Here we determine the identity of unassigned peptides using an ultra-tolerant Sequest database search that allows peptide matching even with modifications of unknown masses up to ± 500 Da. In a proteome-wide data set on HEK293 cells (9,513 proteins and 396,736 peptides), this approach matched an additional 184,000 modified peptides, which were linked to biological and chemical modifications representing 523 distinct mass bins, including phosphorylation, glycosylation and methylation. We localized all unknown modification masses to specific regions within a peptide. Known modifications were assigned to the correct amino acids with frequencies >90%. We conclude that at least one-third of unassigned spectra arise from peptides with substoichiometric modifications.

DOI: 10.1038/nbt.3267

1 Figure or Table

Showing 1-10 of 10 references

high-throughput mutation identification through sequence tagging

  • Dasari, S. et al. TagRecon
  • 2010
Showing 1-10 of 15 extracted citations


Citations per Year

70 Citations

Semantic Scholar estimates that this publication has received between 18 and 198 citations based on the available data.

See our FAQ for additional information.