A map of the restriction targets in yeast 2 micron plasmid DNA cloned on bacteriophage lambda

The 2 micron circular DNA from S. cerevisiae has been cloned on bacteriophage λ. The two forms of circular DNA which exist in equilibrium due to recombination between inverted repeat sequences were separated as stable clones, and a map of the targets for restriction endonucleases EcoRI, HindIII and HpaI was constructed. The circular DNAs isolated from a… CONTINUE READING