A manual method for reducing sugar determinations with 2,2'-bicinchoninate reagent.

@article{Mcfeeters1980AMM,
  title={A manual method for reducing sugar determinations with 2,2'-bicinchoninate reagent.},
  author={R. F. Mcfeeters},
  journal={Analytical biochemistry},
  year={1980},
  volume={103 2},
  pages={
          302-6
        }
}
Abstract A method was developed to analyze reducing sugars manually by use of a 2,2′-bicinchoninate reagent. Potassium phosphate was used to buffer the reagent. Ethylene glycol increased the sensitivity of the assay for several sugars. Sugar determinations can be performed in the presence of borate ion. In comparative assays of a number of monosaccharides and disaccharides, the bicinchoninate reagent was about as sensitive as the Nelson reagent but more convenient to use. The effects of… CONTINUE READING

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