A malachite green colorimetric assay for protein phosphatase activity.

  title={A malachite green colorimetric assay for protein phosphatase activity.},
  author={T. Geladopoulos and T. Sotiroudis and A. Evangelopoulos},
  journal={Analytical biochemistry},
  volume={192 1},
  • T. Geladopoulos, T. Sotiroudis, A. Evangelopoulos
  • Published 1991
  • Chemistry, Medicine
  • Analytical biochemistry
  • A simple and sensitive colorimetric assay for protein phosphatase activity based on the determination of released Pi by an improved malachite green procedure (A. A. Baykov, O. A. Evtushenko, and S.M. Avaeva, 1988, Anal. Biochem. 171, 266-270) is described. Proteins must be removed or stabilized prior to Pi determination with 0.25 N sulfuric acid or 3% (w/w) perchloric acid. Alternatively, to avoid possible acid hydrolysis of phosphate groups from organic compounds during deproteinization, the… CONTINUE READING
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