A malachite green colorimetric assay for protein phosphatase activity.

@article{Geladopoulos1991AMG,
  title={A malachite green colorimetric assay for protein phosphatase activity.},
  author={T. Geladopoulos and T. Sotiroudis and A. Evangelopoulos},
  journal={Analytical biochemistry},
  year={1991},
  volume={192 1},
  pages={
          112-6
        }
}
  • T. Geladopoulos, T. Sotiroudis, A. Evangelopoulos
  • Published 1991
  • Chemistry, Medicine
  • Analytical biochemistry
    • A simple and sensitive colorimetric assay for protein phosphatase activity based on the determination of released Pi by an improved malachite green procedure (A. A. Baykov, O. A. Evtushenko, and S.M. Avaeva, 1988, Anal. Biochem. 171, 266-270) is described. Proteins must be removed or stabilized prior to Pi determination with 0.25 N sulfuric acid or 3% (w/w) perchloric acid. Alternatively, to avoid possible acid hydrolysis of phosphate groups from organic compounds during deproteinization, the… CONTINUE READING
    View on PubMed
    xa.yimg.com
    321 Citations
    Highly Influencial Citations
    8
    Background Citations
    12
    Methods Citations
    75
    Results Citations
    1
    321 Citations
    Citation Type

    Citation Type

    A colorimetric protein phosphatase inhibition assay for the determination of cyanobacterial peptide hepatotoxins based on the dephosphorylation of phosvitin by recombinant protein phosphatase 1
    • 27
    A Sensitive Colorimetric Assay for Protein Phosphatase Activity
    A Sensitive, High-Volume, Colorimetric Assay for Protein Phosphatases
    • 26
    An improved malachite green assay of phosphate: mechanism and application.
    • 69
    • Highly Influenced
    Optimization of a malachite green assay for detection of ATP hydrolysis by solubilized membrane proteins.
    • 6
    • PDF
    Nonradioactive technique to measure protein phosphatase 2A-like activity and its inhibition by drugs in cell extracts.
    • 30
    A Homogeneous, Nonradioactive High-Throughput Fluorogenic Protein Phosphatase Assay
    • 15
    Author ' s personal copy An improved malachite green assay of phosphate : Mechanism and application q
    • PDF
    An automated fluorescence-based method for continuous assay of PP2A activity.
    • 9
    Purification and properties of an acid phosphatase from Lactobacillus curvatus DPC2024
    • 15

    References

    SHOWING 1-10 OF 18 REFERENCES
    A malachite green procedure for orthophosphate determination and its use in alkaline phosphatase-based enzyme immunoassay.
    • 692
    Automated assay of phosphohydrolases by measuring the released phosphate without deproteinization.
    • 34
    A simple micro-assay for inorganic phosphate.
    • C. Penney
    • Chemistry, Medicine
    • Analytical biochemistry
    • 1976
    • 123
    The Malachite green micromethod for the determination of inorganic phosphate.
    • 67
    The further purification and properties of a phosphatase from spleen able to hydrolyze completely the phosphorus of alpha-casein.
    • J. Glomset
    • Chemistry, Medicine
    • Biochimica et biophysica acta
    • 1959
    • 19
    Inorganic and organic phosphate measurements in the nanomolar range.
    • 631
    Automated method for the quantitation of orthophosphate.
    • 14
    An automated assay for glycogen phosphorylase.
    • 38
    A new micromethod for the colorimetric determination of inorganic phosphate.
    • K. Itaya, M. Ui
    • Chemistry, Medicine
    • Clinica chimica acta; international journal of clinical chemistry
    • 1966
    • 688
    Phosphorylase kinase from chicken gizzard. Partial purification and characterization.
    • 15