A mRNA PCR for the diagnosis of feline infectious peritonitis.

  title={A mRNA PCR for the diagnosis of feline infectious peritonitis.},
  author={Fermin A. Simons and Harry Vennema and Jaime E Rofina and J. M. A. Pol and Marian C. Horzinek and Peter J. M. Rottier and Herman F. Egberink},
  journal={Journal of virological methods},
  volume={124 1-2},
A reverse transcriptase polymerase chain reaction (RT-PCR) for the detection of feline coronavirus (FCoV) messenger RNA in peripheral blood mononuclear cells (PBMCs) is described. The assay is evaluated as a diagnostic test for feline infectious peritonitis (FIP). It is based on a well-documented key event in the development of FIP: the replication of virulent FCoV mutants in monocytes/macrophages. To detect most feline coronavirus field strains, the test was designed to amplify subgenomic mRNA… CONTINUE READING