A luminol/iodophenol chemiluminescent detection system for western immunoblots.

@article{Yakunin1998ALC,
  title={A luminol/iodophenol chemiluminescent detection system for western immunoblots.},
  author={Alexander F. Yakunin and Patrick C. Hallenbeck},
  journal={Analytical biochemistry},
  year={1998},
  volume={258 1},
  pages={
          146-9
        }
}
  • Alexander F. Yakunin, Patrick C. Hallenbeck
  • Published in Analytical biochemistry 1998
  • Chemistry, Medicine
  • Chemiluminescent (CL)-based procedures have become a popular method for the highly sensitive immunoblot detection of proteins and nucleic acids. Several chemiluminescent detection systems based on the use horseradish peroxidase (1, 2), alkaline phosphatase (2, 3), or glucose oxidase (2, 4) have been described. Horseradish peroxidase (HRP), a widely used label for immunoassays, catalyzes the oxidation of a cyclic diacylhydrazide, luminol, by hydrogen peroxide to produce a luminol radical (1, 5… CONTINUE READING

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