A luminescent ruthenium complex for ultrasensitive detection of proteins immobilized on membrane supports.

@article{Berggren1999ALR,
  title={A luminescent ruthenium complex for ultrasensitive detection of proteins immobilized on membrane supports.},
  author={Kiera N Berggren and Thomas H. Steinberg and Wendy M Lauber and Joseph A. Carroll and Martha F{\'e}lix L{\'o}pez and Elena Chernokalskaya and Lynn R Zieske and Zhenjun Diwu and Richard P. Haugland and Wayne F Patton},
  journal={Analytical biochemistry},
  year={1999},
  volume={276 2},
  pages={129-43}
}
SYPRO Ruby protein blot stain provides a sensitive, gentle, fluorescence-based method for detecting proteins on nitrocellulose or polyvinylidene difluoride (PVDF) membranes. SYPRO Ruby dye is a permanent stain composed of ruthenium as part of an organic complex that interacts noncovalently with proteins. Stained proteins can be excited by ultraviolet light of about 302 nm or with visible light of about 470 nm. Fluorescence emission of the dye is approximately 618 nm. The stain can be visualized… CONTINUE READING
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